Thomas M.
Iliffe, David J. McAdoo, Carol B. Beyer and Bernard Haber. 1977. Amino
acid concentrations in the nervous system of Aplysia: Neurons with high glycine
concentrations. Journal of Neurochemistry, 28:1037-1042.
The levels of the amino acids glycine, aspartic acid and
glutamic acid were determined in the ganglia and in identified neurons of A.
californica. All of the determinations were done by gas
chromatography- mass spectrometry- selected ion monitoring using
deuterium-labeled amino acids as internal standards. Aspartate and
glutamate concentrations vary 2- to 3-fold among ganglia and individual neurons.
Glycine levels are 3-10 times higher in the abdominal ganglion than in the other
ganglia. This is in large part due to the glycine concentrations in the
abdominal ganglion neurons R3-R14 being about 20 times higher than in the somata
of most other Aplysia neurons. The concentrations of all three
amino acids are several times lower in the muscle than in ganglia, and orders of
magnitude lower in hemolymph than in tissue.
Thomas M. Iliffe, Department of Marine Biology, Texas A&M University at
Galveston, P.O. Box 1675, Galveston, Texas 77553, USA.
E-mail:
iliffet@tamug.edu
Keywords:
Aplysia; neuron; glycine; aspartic acid; glutamic acid.
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